کی تمام اشاعتیں UMAIR MASOOD . Abbottābād ، پاکستان
in-Vitro Site specific DNA Editing Via Restriction Enzyme:
Author: Umair Masood
full paper: https://www.academia.edu/44551733/In_Vitro_Site_specific_DNA_Editing_Via_Restriction_Enzyme
A few years ago, some researchers realized that they could use CRISPR cas9 to gene-editing tools. A part of DNA I like to change if I know the sequence of a gene. I can build a CRISPR that carry a matching code. one inside the cell CRISPR cas9 will scan the DNA until it finds that exact spots. when it does, it cut the DNA right there. now I have the broken gene I can inserted new sequence into the gap. Restriction digestion is a process in which DNA is cut at specific sites. Restriction enzyme bind and cleave double stranded DNA at specific site that main property of restriction enzyme used in DNA editing. Basically, the whole idea is depending upon two things: DNA denaturation and short fragment of DNA is bind to the template DNA and restriction enzyme cleave only double stranded DNA at specific site.
#design MSTN GENE Knockdown plasmid
MSTN basically limits the amount of myostatin.you can produce myostatin, myostatin limits how much mass your skeleton frame can hold so if you got no Mycostatin there is no limit to how big muscle you can get.
Insert a new gene without CRISPR CAS9 Gene editing technique: complete article in academia
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- From my studies of genetics and neuroscience I have come to believe that people fall into four broad personality types - each influenced by a different brain chemical: I call them the Explorer, Builder, Director, and Negotiator
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- The power of biological weapons is ten times more than the nuclear power. Unless we act fast with an open mind, any one of them can extinct the human race
Water memory and Biological Application:
Author: Umair Masood
water memory is ability of water to retain a memory of solute particle after large serial dilution. The role of water memory and electromagnetic waves (EMS) not only theoretical interest but lead to many molecular biology applications. On 13 July 2005 Jacques benveniste and his team detect an electromagnetic signal in water dilution, DR.Jamal aisan his team member observes for first time increasing the frequency of recorded electrical signal emitting by some high dilution of filters of bacteria this was the beginning of water memory research. Soon discover that DNA is a main source of electromagnetic signal in water. The diversity of the DNA sequences emitting EMS does not show any clue however we studies HIV infected patient the electromagnetic waves is emitting by HIV DNA. We detect electromagnetic signal filter at 100 nm pores produce by DNA by intracellular bacterium found in red blood cell these bacteria electromagnetic signal was found to produce in part by human DNA sequence combine in strongly related with bacterial DNA. The same DNA sequence belonging to the chromosomal DNA of the same patient never produce electromagnetic signal. However same sequence was present in red blood cells of someone healthy one
A formerly reported an experiment show that the ability of electromagnetic signal production can be transmitted from tube 1 contain at emitting DNA dilution to tube 2 of water provided the system is excited over night by electromagnetic waves approximately 7Hz.Tube 1 transmitted to the water in tube 2 which cannot have any trace of DNA. The emission of electromagnetic waves exposed tube is resonance phenomena on external waves input. The electromagnetic signal carry specific information of the initial DNA as indicating retrieving the DNA by PCR in the recipient tube. Electromagnetic signal was recorded as a digital file and sent via internet. And University of Göttingen using a record file. The digital file was converted to analog and was amplified. The current was connected to solenoid tube of water inserted in the solenoid and in this way was submitted to the induced modulated magnetic field for one hour. Then pcr ingredient were introduce in water from the tube after 40 Pcr cycle the original DNA was detected as show by a specific band in gel electrophoresis of the expected molecular weight.
Insert a new gene without CRISPR CAS9 Gene editing technique using DNA repairing mechanism:
Author: Umair Masood
A few years ago, some researchers realized that they could use CRISPR cas9 to gene-editing tools. A part of DNA I like to change if I know the sequence of a gene. I can build a CRISPR
that carry a matching code. one inside the cell CRISPR cas9 will scan the DNA until it finds that exact spots. when it does, it cut the DNA right there. now I have the broken gene I can inserted new sequence into the gap.Insert a new gene without Crispr system technique is a combination of two methods: restriction digestion and DNA repair method.Restriction digestion also called restriction endonuclease is a process in which DNA is cut at specific sites.In restriction digestion find the gene that need to be remove from DNA to replace the new gene by using restriction digestion enzyme.In order to analysis restriction digestion site we can use a different bioinformatic tools.DNA repair is a process in which cell identifies and corrects damage DNA using Template DNA and enzymes.During the repair process cell is looking for Template DNA to figure out how to fill in the gene that was cut.After restriction digestion cell repair machinery fixes the break by using our Template DNA and then cell has a new DNA sequence.
DNA digital data storage and Random DNA seq convert into exact given binary information:
Author: Umair Masood
can our knowledge is survive? the printed page will decompose and hard drive storage will be crash.But something inside us called DNA that can more powerful chemical limitation..DNA storage already our biological information from height to skin tone it program and run our entire body.DNA sequence is made of A.T.C.G first step in process is to turn the data we want to storage into the DNA sequence of those four molecules.Than sequence are synthesized into the actual DNA.than read the sequence and turn back into the data.The data we want to storage into the DNA is always given in binary form we are converting binary sequence into the DNA sequence.
Random DNA seq convert into exact given binary information:
- Random DNA sequence convert into the exact given binary info technique is depend upon binary to binary DNA information technique in which the data we want to storage into the DNA, DNA bases binary is change according to desire information for example
our sequence is A T T G C
Given info binary is 00 10 10 00 11
- In this case we can change the binary representation of DNA bases and given sequence of binary is
- change in binary representation is a software method the reading machine change the binary according to input binary representation